FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Kn-0_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Kn-0_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27368379
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55950.049.4208146
CCCGTCC75200.046.8194733
CACCCGT85200.041.44761331
CGTATGC67150.041.28280344
ACCCGTC88250.040.45138532
TCACCCG90850.039.17815430
GTATGCC73850.038.0582445
GTCACCC95200.037.20429629
ACGTCTG104800.036.33459515
ATGCCGT76500.035.6412247
GCACACG107900.035.55063611
CACACGT113150.033.90070312
TCGTATG79550.033.08826443
ACACGTC125050.030.6466613
TGCCGTC90250.028.8537548
CGTCTGA135750.028.1536516
CCGTCCA125500.027.07892234
CACGTCT148900.025.71424314
GAGCACA155750.025.2128729
AGCACAC156200.024.84890210
Produced by FastQC (version 0.11.5)