FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Kn-0_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Kn-0_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14784815
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC372400.25188005396076985TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG66200.059.11013846
GTATGCC72550.054.27436445
CGTATGC73150.052.68100744
GCACACG107500.051.8628211
ACGTCTG107300.051.79657415
ATGCCGT74450.051.0550747
CACACGT110500.050.4862812
ACACGTC116200.047.9498413
TCGTATG74050.047.787243
TGCCGTC80500.047.0004648
CGTCTGA123450.045.16218616
CACGTCT126700.043.92085614
GCCGTCT79700.043.29912649
TCGGAAG134250.041.7714233
GAGCACA135000.041.635339
AGCACAC135550.041.5695310
CTCGTAT74450.039.9141642
GATCGGA139600.039.7968751
ATCGGAA146850.038.139552
TCCAGTC150600.036.85896325
Produced by FastQC (version 0.11.5)