FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Ge-0_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Ge-0_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18159842
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25100.040.16072546
CGTATGC35050.028.9600944
GTATGCC35550.028.74945445
ATGCCGT38100.027.00834547
TCGTATG44250.022.93926243
ACGTCTG47250.022.88796215
GCACACG47800.022.40525811
TGCCGTC46250.021.94632748
CACACGT50350.021.68742612
GCCGTCT51500.019.09699849
ACACGTC58400.018.45825813
CTCGTAT51650.016.9419942
CGTCTGA71500.015.369966516
CACGTCT72700.014.97192614
AGCACAC78450.014.81154710
GAGCACA80100.014.6812169
TCGGAAG84450.013.0977453
GCGGGAT26000.012.6567151
GGGCGCT4802.7048372E-911.6693121
GCGGACT15450.011.5560171
Produced by FastQC (version 0.11.5)