FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Ge-0_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Ge-0_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17230340
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25500.038.84287648
ACGTCTG56850.031.76704215
GCACACG58000.031.61983511
CGTATGC33750.030.2816346
GTATGCC33600.030.10396647
CACACGT62750.029.4492312
ACACGTC66800.027.03519613
ATGCCGT36500.026.94447749
TCGTATG41300.025.16983445
CGTCTGA80000.022.88069716
ATGTCAG77100.022.78770634
CACGTCT80800.022.69747214
AGCACAC88100.021.41227710
GAGCACA92000.021.1512959
GTCAGAA83550.020.94558536
TGCCGTC45600.020.18568250
ACATGTC90550.020.17574332
TGTCAGA89950.019.80514335
TCGGAAG93800.019.703193
GTCACAT92000.019.36343829
Produced by FastQC (version 0.11.5)