FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Ge-0_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Ge-0_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13640917
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC148730.10903225934150909TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27100.046.75375746
GTCACGC31300.042.6025529
CGTATGC33200.039.534444
GTATGCC35550.036.5270645
TCACGCC37450.036.16713730
ATGCCGT35550.035.7386347
ACGTCTG42450.032.4017315
CACGCCA43850.031.44717231
TCGTATG42350.031.15859843
TGCCGTC42650.030.03525448
GCACACG47250.029.33266311
CACACGT48200.028.75400412
GCCGTCT46800.026.84797349
CTCGTAT46850.026.82108942
TATCTCG47500.026.6014439
ACGCCAA52900.026.06707432
CGCCAAT53150.025.81248333
ACACGTC53850.025.54221713
AGTCACG55050.024.6676528
CGTCTGA64750.021.45865616
Produced by FastQC (version 0.11.5)