FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Ct-1_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Ct-1_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16773392
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20700.033.9860648
CGTATGC29050.025.06079946
GTATGCC29500.024.20385747
ATGCCGT31900.022.38214149
ACGTCTG40000.020.03687115
TCGTATG37100.019.8118245
GCACACG44250.017.55892811
TGCCGTC41350.017.01281750
TATCTCG42800.016.4378241
CACACGT49700.016.05602512
CACACTG51650.015.314136531
CTCGTAT47000.015.11758244
GCCGTCT49350.014.75152951
ACACGTC55150.014.53280813
CACGTCT65650.012.47496714
CGTCTGA64850.012.35890216
AGCACAC69250.012.33188910
GCGGGCC3701.5240585E-712.3000271
GCCGGAT31650.012.0563891
GCGGGCT12250.012.0026651
Produced by FastQC (version 0.11.5)