FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Ct-1_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Ct-1_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26146282
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT342410.13095934634224476TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60300.048.52470448
CGTATGC69850.041.5907246
GTATGCC74400.039.70491447
ACGTCTG96300.036.5254415
ATGCCGT81250.036.0117649
TCGTATG78650.034.8902245
GCACACG104350.034.00950611
TCCGTAT95700.033.72170337
CACACGT109600.032.25260512
GTTCCGT104350.031.7967235
TGCCGTC94000.030.7919150
ACACGTC118600.029.68723713
TATCTCG89300.029.67209641
TTCCGTA112000.029.500436
CGTATCT91250.029.45979139
CTCGTAT92750.027.73744444
GCCGTCT100950.027.2504451
CCGTATC108250.027.03204338
AGTTCCG123000.026.97463634
CGTCTGA135850.026.20079416
Produced by FastQC (version 0.11.5)