FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Ct-1_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Ct-1_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14411721
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32450.047.45892746
CGTATGC36850.041.98361644
ATGCCGT41450.037.06900447
ACGTCTG53550.036.8618415
GTATGCC43150.036.25861745
GCACACG57400.034.38951511
CACACGT58000.034.03376812
TCGTATG45350.032.571543
ACACGTC65300.030.44356513
TGCCGTC49850.029.98024448
CACAGTG70850.027.1689433
CGTCTGA73950.026.92960216
CACGTCT75500.026.4697314
GCCGTCT53300.026.26586249
CACACAG77000.025.27166731
AGCACAC80600.024.92477210
ACACAGT80150.024.32212632
TCGGAAG83950.023.7652933
GAGCACA86100.023.7390889
GTCACAC93900.021.17079229
Produced by FastQC (version 0.11.5)