FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Col-0_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Col-0_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13887876
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17850.034.70707346
CGTATGC23000.026.63163644
GTATGCC26500.023.5104145
ATGCCGT27050.022.77309847
ACGTCTG34750.020.44497915
TGCCGTC32400.019.01258948
GCACACG38500.018.36261411
TCGTATG33950.018.24850843
GCCGTCT35200.017.20155149
CACACGT42950.016.62305612
ACGGTAG41700.016.6179632
ACACGTC46350.015.32816513
CTCGTAT37650.015.15362542
GTCACGG47350.014.85683229
AGTCACG48150.014.24650528
CACGTCT54300.013.406314
GGTAGCA51300.013.09901634
CGTCTGA54850.012.95283516
GCGGGCT10550.012.6092941
CGGTAGC57750.012.30252333
Produced by FastQC (version 0.11.5)