FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Col-0_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Col-0_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25294159
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT394420.1559332334393881TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG66400.052.13105448
GTATGCC77550.044.7713247
CGTATGC79700.043.87148346
ATGCCGT86850.040.3392149
TCGTATG91300.037.9142445
ACGTCTG99250.037.55536315
TGCCGTC95500.036.13564750
GCACACG106450.034.95001211
CTCGTAT91850.033.87717444
GCCGTCT106950.032.1360251
CACACGT117250.031.8798612
ACACGTC121700.030.54160913
AGCACAC135500.028.12833210
CACGTCT132850.028.03079814
ACAGTCA136300.026.78178832
CACAGTC136450.026.7269131
GAGCACA146250.026.3720389
CGTCTGA143100.026.21856116
TCGGAAG150150.025.0374053
GTCACAG157100.023.32527529
Produced by FastQC (version 0.11.5)