FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Col-0_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Col-0_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14290325
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC151660.10612774726956875TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30300.050.7106346
CGTATGC38050.040.8422844
GTATGCC40150.038.6183645
ATGCCGT40800.037.5740547
TGCCGTC45050.034.3399848
ACGTCTG49000.033.92714715
TCGTATG48550.032.2977343
GCACACG52100.031.90878111
ACTGACC54300.029.39133632
GCCGTCT52550.029.038649
CACACGT58650.028.64339612
CACTGAC55650.028.55245831
ACACGTC58400.028.2866813
CTCGTAT49950.025.15603342
CGTCTGA70400.023.81291816
CACGTCT70250.023.66464814
AGTCACT70250.023.2662528
AGCACAC74900.022.70964210
GAGCACA77600.022.4156079
TCGGAAG75450.022.083013
Produced by FastQC (version 0.11.5)