FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Bur-0_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Bur-0_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23831826
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC598160.2509920977100118TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG106000.056.56366746
CGTATGC113600.052.78157844
GTATGCC114500.052.51811645
ACGTCTG137250.049.34237315
ATGCCGT120450.049.02165247
TCGTATG125600.047.29335443
GCACACG150000.045.12569811
TGCCGTC137050.042.93064548
ACACGTC157500.042.86538313
CACACGT160200.042.2303212
GCCGTCT136600.041.7385449
CTCGTAT119100.039.26448442
CACGTCT174500.038.95012314
AGCACAC177500.038.46943310
CGTCTGA178250.038.1107416
GTCACCT173900.037.85683429
GAGCACA186250.036.8125539
TCGGAAG195750.034.671023
GATCGGA203500.032.9920581
AGAGCAC211700.032.221728
Produced by FastQC (version 0.11.5)