FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Bur-0_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Bur-0_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13859041
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29050.046.26609446
GTATGCC35000.039.1014445
CGTATGC34400.038.76624344
ACGTCTG47450.033.70797715
ATGCCGT39600.033.14372647
TCGTATG40900.031.23643143
ACTTAGG51850.030.30762132
GCACACG54000.029.74948711
CACTTAG55300.028.41702531
CACACGT58200.027.5423812
TGCCGTC47300.027.23026748
TTAGGCA58000.027.15421334
ACACGTC59500.026.99943713
TCACTTA62350.025.70907430
CACGTCT64350.025.07310914
AGTCACT65250.024.45934928
TAGGCAT61750.024.1456835
CTCGTAT44150.023.86340142
AGCACAC70050.023.28271510
CTTAGGC68750.023.21372633
Produced by FastQC (version 0.11.5)