FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Bl-1_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Bl-1_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18226241
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT254550.1396612718991261TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG43200.051.04218748
CGTATGC51350.042.9417546
GTATGCC51950.042.24308447
CGTGGCC58150.040.685533
ATGCCGT57650.038.1261849
ACGTGGC66050.035.66039332
GCCTTAT65750.034.8155737
TCGTATG62050.034.63485345
CACGTGG69900.034.54762631
TGCCGTC67900.032.57684750
GTCACGT74050.032.56455629
TCACGTG78200.030.88071830
TATCTCG64900.030.31063741
CTCGTAT65800.029.94818344
GCCGTCT72650.029.86888551
AGTCACG83800.028.98486128
GTGGCCT89950.026.41883534
GGCCTTA89950.026.07030336
ACGTCTG98850.025.1734515
TCTCGTA92700.021.22042543
Produced by FastQC (version 0.11.5)