FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Bl-1_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Bl-1_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21587965
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC297830.1379611278784267TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG51150.052.484146
ACGTCTG103650.044.5720415
GTATGCC63850.043.3059245
CGTATGC62950.041.36788644
GCACACG113750.040.70702711
ATGCCGT64300.039.57258647
ACACGTC118700.039.12714413
CACACGT118900.039.00245712
CACTAGC128750.035.25726731
CACGTCT133000.035.0781314
TCGTATG70400.034.3054843
ACTAGCT133000.033.86731732
CGTCTGA137150.033.8377616
TATCTCG84150.033.7748239
AGTCACT133950.033.7580828
TGCCGTC75450.033.1678148
AGCACAC140300.033.15310710
TAGCTTA135900.033.0156234
TCACTAG138050.032.85666730
GTCACTA139600.032.14093829
Produced by FastQC (version 0.11.5)