FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Bl-1_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Bl-1_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17102901
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC189140.11058942573543516TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32300.048.22096346
GTATGCC42000.038.00090445
CGTATGC41250.037.5044744
ATGCCGT43650.035.92254347
ACGTCTG61950.034.12301315
CCGATGT60450.033.92788733
GCACACG63850.033.32715211
CACACGT67700.031.48339712
TGCCGTC51000.031.01998348
ACACGTC71750.029.60872813
TCGTATG50450.029.48602943
TATCTCG54650.028.24403839
GATGTAT75750.026.19787835
CACGTCT84500.025.38957614
GCCGTCT58800.025.35715349
CGATGTA81650.025.24750334
AGCACAC87900.024.76621210
ACCGATG86450.024.57426332
CGTCTGA86900.024.56754716
CTCGTAT58200.024.35632342
Produced by FastQC (version 0.11.5)