FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Akita_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Akita_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14758285
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT290730.1969944339738662TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC48200.057.5087533
TATGCCG48000.055.48951748
GTCCGCA52050.052.91895334
ACGTCCG54700.050.99507532
GTATGCC53900.049.41552747
CGTATGC54450.048.98132346
CCGCACA56250.047.537436
TCCGCAC60050.045.86943435
ATGCCGT58850.045.3778749
CGCACAT56900.045.27281637
TCGTATG61200.042.66363545
GTCACGT67100.041.57166729
ACGTCTG68750.041.38805815
GCACACG70950.040.59827411
TGCCGTC66000.039.9845350
CACACGT75650.038.3070412
GCCGTCT68600.037.90760451
AGTCACG74900.037.335928
ACACGTC78050.036.8600713
TCACGTC79250.035.1097730
Produced by FastQC (version 0.11.5)