FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Akita_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Akita_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26936369
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC774220.2874255249473305TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG125150.057.66874746
CGTATGC132200.054.7264644
GTATGCC137050.052.6614945
ATGCCGT141850.050.73022547
TCGTATG144550.049.39716343
ACGTCTG154450.049.2629615
GCACACG158650.047.87109411
TGCCGTC152100.046.98918548
CACACGT165900.045.86320512
GCCGTCT160850.044.1710349
ACACGTC176450.043.2796313
CTCGTAT146900.042.9601242
AGTCACG173600.042.5796328
GTCACGA174300.042.06719629
CGATCAG175750.041.6799133
CACGTCT194600.039.22495714
CGTCTGA194850.039.17448416
AGCACAC208550.036.93709610
TCACGAT201650.036.63934730
GAGCACA213500.036.0316629
Produced by FastQC (version 0.11.5)