FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Akita_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Akita_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15642000
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC229110.1464710395090142TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45500.053.61598646
CGTATGC50650.048.37264644
GTATGCC53900.045.52028345
ATGCCGT55600.043.8761147
TCGTATG60650.040.2240743
TGCCGTC60950.039.9098348
ACGTCTG63800.039.82663315
GCACACG65450.039.41082411
CACACGT67650.037.97408312
GCCGTCT66850.036.1247949
CTCGTAT60250.033.86857642
ACACGTC76400.033.67087613
CGTCTGA83600.030.77079216
CACGTCT85550.030.23315214
AGCACAC92100.028.72895210
GAGCACA96750.027.384279
TCGGAAG97500.026.566273
TCACGAT88250.026.5327935
GTCACAT102250.024.78180329
GATCGGA103750.024.663751
Produced by FastQC (version 0.11.5)