FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132024145
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4210100.31888863964996705No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3594650.2722721665798328No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3176760.24061962302425818No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1839800.13935329783805833No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1806910.13686208685540058No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1489040.11278543027110684No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1372890.1039877970806022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA682900.066.4074551
CCGGGGA353650.065.888791
CCGGAAA999250.062.343011
CGGGGGA893600.058.6446951
CGCGGGA70450.057.9998631
GCGGGAA298350.057.575621
ATTTAAA262550.056.504571
GCGGGGA201100.056.307351
CCCGGGA68000.052.6749151
CCGGGTA25800.049.5347861
CGCGGAA64600.049.3226051
GCCGGGA73950.048.9575271
CGGGGTA40000.047.5310021
TTTGAAA119050.047.335772
GGGGAGA1534800.044.183353
ACGGGAA72150.044.161331
AATTGAA27750.043.782811
CGGGGAG370500.043.551762
CGGGAAA2600900.043.3453331
GCGGGTA12200.043.0495451
Produced by FastQC (version 0.11.5)