FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_6BRS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_6BRS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636670
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA573833.5060824723371233No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA518603.168628984462354No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206391.2610361282359914No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200701.2262704149278718No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185451.1330934152883598No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169601.0362504353351623No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148320.9062303335431088No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102650.6271881319997311No Hit
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90040.5501414457404364No Hit
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47130.28796275364001295No Hit
GGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30770.18800369042018245No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC26730.16331942297469865No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC25650.15672065841006433Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC25250.15427667153427388No Hit
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA23070.14095694306121576Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21790.13313618505868624No Hit
CGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18120.1107126054733086No Hit
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC17690.10808531958183384Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17020.10399164156488479No Hit
GGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16610.10148655501719957No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGT150.002223436570.0129923
TTAATAT150.002224514570.0044315
AAAGCTG150.002225323369.9980238
CGCTGGG150.00222559369.995881
CATGAGT252.8425275E-669.995886
GGCTGGG150.00222559369.995881
GCAATGT150.00222559369.995884
GCGGAGT150.00222559369.995881
GCAATGA150.00222559369.995884
GCGTGGG150.00222559369.995881
GCCAGGG301.02498234E-769.995881
TCAGCCA150.00222559369.9958855
GCAGTGA150.00222559369.995882
GTGGGAG252.8425275E-669.995883
GCGCTCT150.00222559369.995883
GCCATCT252.8425275E-669.9958869
CCGAGAT150.00222559369.995881
GCCGTAA150.00222559369.995881
CGGCATG150.00222559369.9958870
GCCGATG301.02498234E-769.995881
Produced by FastQC (version 0.11.5)