FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_6BFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_6BFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7700593
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1165951.5141041735357263Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA851441.1056810819634282No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA688750.894411638168645No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC682320.8860616318769218Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470350.6107971165337527No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG449430.5836303775566375Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG347270.45096526981753227Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA311030.40390395908470944No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA304970.3960344352701149No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA282220.36649125593314696No Hit
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC268600.3488043063696523Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA250180.3248840706163798Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248150.3222479099986196No Hit
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT232710.3021975060881675Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT210790.27373216582151527Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA204960.26616132030351425Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA198480.2577463839473142Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA190410.24726667154075016Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT183360.23811153244951397Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT177510.23051471490572217Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC167580.21761960410061928Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162020.21039938093079324No Hit
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT154600.2007637593624283Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC151300.19647837510695607Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC143040.18575192845538Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA141420.18364819436632998Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140820.18286903359260773No Hit
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA139240.18081724355513917Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA138740.18016794291037067Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC133700.1736229924111039Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG127940.1661430489833705Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA121330.1575592944595306Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT120560.15655937146658708Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112050.14550827449262674No Hit
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT111400.14466418365442765Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC108510.14091122592766556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC102260.132794967868059Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC93140.12095172410748108Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT92930.12067901783667828Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC89430.1161339133232986Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG85590.11114728437147632Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC80370.10436858564009291Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG79640.10342060669873086Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT79020.1026154738992179Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGGA207.9258876E-570.0099726
GTTGGGG207.926652E-570.00860625
CGACGCG150.002225562569.998613
GGGGCGT252.8428676E-669.998613
TTACTGG150.002225619769.998157
GGCCCCT150.002225619769.998151
GCCGCAG401.3460522E-1069.998151
GCCTGCT150.002225619769.998151
GCCCTTG252.8429768E-669.998151
GGCGATT150.002225619769.998151
CGCGTGG252.8429768E-669.998151
GCCGTAG252.8429768E-669.998151
TTTAGTG150.00222567769.99769612
GCAAGTG207.932773E-569.9976962
CATGCTT150.00222567769.99769613
CGTGCTT150.00222567769.9976962
TACATTT150.00222567769.99769669
CGCTCTA150.00222567769.9976962
GCGATTA150.00222567769.9976962
TTAGTGG150.00222567769.99769613
Produced by FastQC (version 0.11.5)