FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_6ARS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_6ARS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872442
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA203412.331501692949216No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161011.8455095009181126No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120001.3754496000880287No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76100.8722642880558249No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67510.7738050208495235No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65320.748703065647917No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53220.6100118976390407No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC49040.5621004032359744Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41740.47842721923061937No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC30870.3538344096226454Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30490.3494788192223667No Hit
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24270.2781846816178038No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG19010.2178941408139452Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC15330.17571368641124568Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG15270.17502596161120165Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14450.16562705601060013No Hit
CCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13660.15657201281002062No Hit
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA13350.15301876800979322Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA13060.14969476480958047Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12160.13937889280892024No Hit
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA11400.13066771200836272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT11240.12883377920824535Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT10910.12505129280800328Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA10820.12401970560793726Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10420.11943487360764383No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC10150.11634011200744576No Hit
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT9640.11049445120707165Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC9120.10453416960669018Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA8920.10224175360654347Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG500.069.996911
GCGGGAT252.841165E-669.996911
GCAGGGA700.069.996912
TGGAGAG252.841165E-669.996914
GCCGGAT252.841165E-669.996911
CGCAGGA252.841165E-669.996911
TTTTGAG301.0243093E-769.99692
GAAACTG150.002225114569.99696
AACTGAC150.002225114569.99698
GCGAATA207.929283E-569.99692
CGAAGTG150.002225114569.99693
CCAGACT150.002225114569.996949
GAAGATG150.002225114569.99694
CGTAGCA150.002225114569.996948
CCGTTCC150.002225114569.996970
GCGGAGT301.0243093E-769.99691
GCGTGGG207.929283E-569.99691
AGAACAT150.002225114569.99696
GCCAGGG150.002225114569.99691
GTGGGAG550.069.99693
Produced by FastQC (version 0.11.5)