FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_6AFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_6AFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8528904
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1804802.116098387319168Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1104451.2949495034766483Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG917871.0761875148319175Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA793910.9308464487347965No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG656870.7701692972508543Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA637100.7469892966317829No Hit
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC460350.5397528216990131Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA429070.5030775349329761No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT421440.4941314851239971Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA407830.47817398343327583Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA377220.44228426067405613Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT349950.41031063311300026Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT315900.3703875667963903Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA302840.35507493108141447No Hit
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA300970.3528823867638796Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA296740.3479227811686003No Hit
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA279530.3277443385457264Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA254980.2989598663556302No Hit
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC254360.29823292652842615Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA251440.2948092744390135No Hit
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC250540.2937540392059753Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT245440.28777437288542584Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA237820.2788400479123695Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA233590.2738804423170902Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA227640.26690416494311575Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA226060.26505164086733773Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT209960.2461746550318775Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT198560.23280834208006096Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG187970.22039174083797872Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT181140.21238367790281143Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA175530.20580604495020696Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC164320.19266250388092068Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC161220.18902780474490039Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC154350.18097284246604253Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150460.1764118812921332No Hit
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA141900.16637542174234812No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG140900.16520293815008352Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA138600.16250622588787492Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC135540.1589184260955452Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT131730.15445126360901706Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC131580.15427539107017735Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG130440.15293875977499571Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116860.13701643259204233No Hit
CGGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG116650.13677021103766673Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC112280.1316464577394704Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC111330.13053259832681902Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC109850.12879732261026738Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC106920.12536194568493209Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC104930.12302870333632551Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC103910.1218327700722156Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT102380.12003887017605075Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC100780.11816289642842738Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA99720.11692006382062689Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99470.11662694292256075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97480.11429370057395417No Hit
GGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC94790.11113971971076236Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA89700.1051717782261355Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG85660.10043494451338647Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG150.002225321370.0005938
TTAGGTT207.933046E-569.99737
GAATGTG252.8432096E-669.99734
CGACGTG207.933046E-569.99733
GGTTTCT150.002225735269.99733
GCTTGTC207.933046E-569.99733
GAGACTG150.002225735269.99734
TCTACCT150.002225735269.99738
GGGCCAT150.002225735269.99733
AGTAATT150.002225735269.99736
ATTTAGG150.002225787169.9968951
GCCAGTG301.0254007E-769.9968951
ATTTGTG207.933276E-569.9968951
TTTCTAT252.8433096E-669.99689511
GCCTGCT207.933506E-569.996482
GGTGCTC150.002225838869.996482
GCCTAGC207.933506E-569.996482
GCAGATA150.002225838869.996482
CGCTTGT301.0254371E-769.996482
TGCCGTG252.8434079E-669.9964870
Produced by FastQC (version 0.11.5)