FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_5BRS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_5BRS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247138
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA738443.2861355199369155No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA718513.1974449277258445No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA262181.1667285231258606No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA258431.150040629458449No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA232591.0350499168275378No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220450.9810256423949041No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA196620.8749796407697257No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA144000.6408151168286059No Hit
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123060.5476299185897795No Hit
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66540.2961099852345517No Hit
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC64410.2866312616314619Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT52480.23354150924420305Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC48790.21712062187547004No Hit
CGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC45850.20403731324021931Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA44900.1998097135111417Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41540.18485736078514092No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC40200.1788942201146525No Hit
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36730.1634523558410743No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT32800.14596344327762692Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC29680.13207911574634046Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG27550.12260039214325065Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27450.122155381645453No Hit
GGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26760.11908480921064928No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG25310.11263215699258346Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA24070.10711402681989268Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC23410.10417695753442824Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA23170.1031089323397139No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG22510.10017186305424945Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACATG207.9308986E-569.9988663
CTGAGCC150.002225521569.997342
AATACTA150.002225521569.997344
CTCTCGA150.002225717869.9957452
ACGTGCC150.002225717869.9957451
CTGGGAG207.9335216E-569.9941943
GCAATGT207.9335216E-569.9941944
GTGGGAG252.8432787E-669.9941943
CGATGGA207.9335216E-569.9941943
GGCAGTA207.9335216E-569.9941943
GCCGAGT900.069.9941941
CCGATGG207.9335216E-569.9941942
GGCGGTG207.9335216E-569.9941941
CCTGGAA207.9335216E-569.9941942
AGCTTGC150.002225914369.9941970
GCAGTGA150.002225914369.994192
CTAACAT150.002225914369.9941962
AGGCTGA150.002225914369.9941950
GTCTTCT150.002225914369.9941970
CGGCCTT150.002225914369.9941970
Produced by FastQC (version 0.11.5)