FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_5BFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_5BFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14682172
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1786011.2164480841118057Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1418200.9659333782494851No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1136510.7740748439672277Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1134460.772678592785863No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1095540.746170253284051Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG784290.5341784580646515Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA752010.5121926101941865No Hit
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA669450.4559611479827371Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC581740.3962220303644447Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA561610.3825115248615804No Hit
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT537410.36602894994010426Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA533630.3634543989812951No Hit
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA494880.3370618461628157Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA476990.3248770004873938No Hit
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA474270.3230244135540709Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA460970.31396580832863147Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA457690.31173180643844794Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450770.3070186073286704No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT428460.2918233078865988Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA385620.26264506368676244Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC340300.23177769610654336Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT319740.2177743184046611Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT311190.21195092933116436Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA309790.21099739193901285Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC293140.19965710795378233Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA279670.19048271604501024Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG271310.18478873561759118Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT266710.1816556841862362Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA259630.17683350937449854No Hit
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC259560.17678583250489097Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA254640.17343482966961565No Hit
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA232610.15843023770597428Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT222750.1517146100726786Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT219530.14952147407073013Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC216250.14728747218054658Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC216080.1471716854972139Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC204930.13957744126686433Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA202180.13770442138942385No Hit
CGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC197970.1348369982315968Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG196860.13408097929924812Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC188320.12826440120712385Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG184510.12566941730419723Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA183140.12473631285616325Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG180780.1231289212522507Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC169570.11549381113366605Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC169270.11528948169249073Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT168300.11462881649935719Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC167560.11412480387779136Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC155500.10591076034254332Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG149650.10192633623962448Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTTA207.9319245E-569.9996441
ACGCTTT150.00222559369.9986969
GTATATT150.00222559369.9986969
TTTGCAT150.00222559369.9986966
TATACTT207.93246E-569.9986969
ATCCTTT353.7180143E-969.9984416
TTCTATC353.7180143E-969.997512
CCTAGCG150.002225743469.997548
TTTAGGA150.002225773469.997251
GGAGCAT150.002225773469.997254
GCGAATT252.8433242E-669.997252
GCGCGTT207.9332625E-569.9972570
TAGGTTA455.456968E-1269.997258
CGTGAGT207.9332625E-569.997253
TGGCGTC150.002225773469.9972570
GGATCTT150.002225773469.997254
TATATTG207.9332625E-569.9972570
TTTCCGG150.002225773469.9972570
CGCTGGG2050.069.997251
CGCTGAG150.002225773469.997251
Produced by FastQC (version 0.11.5)