FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_5ARS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_5ARS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245241
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA376871.67852805110899No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA307231.3683609020145275No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA208280.9276509737707445No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC184360.8211145262357137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140860.6273714046732622No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132630.5907160968466192No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131330.584926072524063No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC113720.5064935122777465Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA106080.47246598472057116No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG96150.42823910662597026Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75830.33773657259955614No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG73100.3255775215221885Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64710.2882095953173846No Hit
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC63550.28304311207571925Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA55940.24914920046444902Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA51460.2291958858759483Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49680.22126800641891006No Hit
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT48210.21472082506955822Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA46430.20679294561251998Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA38690.17232003156899414Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC38090.16964771265089137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA35460.1579340480598742Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA35060.15615250211447235Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT34930.15557349968221673Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34290.15272302616957378No Hit
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT32510.14479514671253554Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC30650.13651095806641694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC28340.12622253023172122Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA28330.12617799158308618Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT28000.12470821617812965Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG27500.12248128374637733Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC26540.1182055734774129Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT25160.1120592399657765Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24880.11081215780399521No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC24190.10773899104817701No Hit
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC24040.1070709113186513Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC23470.10453220834645367No Hit
GGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23390.10417589915737331No Hit
CGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23380.10413136050873827No Hit
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA22900.10199350537425603Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGG150.002224717870.0036838
GGCACTT150.00222550469.9974445
GGAACTT150.00222550469.9974443
TTTCTAG150.00222550469.99744415
ATGATCA207.931696E-569.997449
TAGAGGG150.002225700569.9958810
GCCTTTT207.932571E-569.995882
GCGACGG353.7180143E-969.995882
AATTTGA301.02514605E-769.995881
CAAGTTT150.002225700569.9958867
CGAGTTT150.002225700569.9958869
CGTGAGG150.002225700569.995882
GGCAAGT150.002225700569.995881
GCCGGGT150.002225700569.995881
AAACGTT150.002225700569.9958870
GCCGAAT800.069.995881
CTTATCC150.002225700569.9958850
AATTGAG150.002225700569.995881
TATTCCT252.8428694E-669.9958811
TTTGGAG401.3460522E-1069.995882
Produced by FastQC (version 0.11.5)