FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_5AFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_5AFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10262784
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1709431.6656591427823095No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450071.412940192446806No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1083061.0553276771683007Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA874120.8517376961261194No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC678930.6615456390780513Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA664460.6474461510638829No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG654000.6372539848836339Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA645380.6288547045324153No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA541140.5272838247399536No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA533890.5202194648157848No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG486350.473896751602684Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT399470.3892413598493352Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT354410.34533514492753625Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA345490.33664354623462794No Hit
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC322010.3137647640250443Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA298190.2905546876948789No Hit
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA246030.23973027201975605No Hit
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA231540.22561129611614159Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA227470.22164551061388413Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA223480.21775767666941054Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT189390.18454056910873307Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC187180.18238715732495198Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG180980.17634591159669735Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT179230.17464072127017385Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC176980.17244833370750082Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA175370.1708795586070992Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA174510.17004157936092193Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA163730.15953760694953728No Hit
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC159990.15589337162313852Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC151220.1473479321010751Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG141020.13740910848362395Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA140910.13730192509167102Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT134570.13112426413729453Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT129540.12622305994162988Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA126030.12280293534385991Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT125490.12227676232881837Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG121310.118203793434608Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC119370.11631346815834769Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC117320.11431595949013444Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA116630.11364362730424805Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC113660.11074967572151963Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112510.10962912207837562No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC110680.1078459801940682No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC104350.10167806318441468No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG150.002225588569.9985738
CGACGCG252.8432733E-669.997213
GTCCTAC150.002225760469.997213
TGTGGCT150.002225760469.997219
CTCGACG207.9331745E-569.997214
AGGCTAG150.002225760469.997215
TACCGAG150.002225760469.997219
GGCCTGT150.002225846569.996521
CGTGGCT252.8435206E-669.9961852
CGTATCT207.933749E-569.9961852
CGTGCGT150.002225889469.9961852
CGAGGTC150.002225889469.9961852
GCCTAGG207.933749E-569.9961852
GCAGAGT353.7198333E-969.9961852
GCGAGGT207.933749E-569.9961852
GCCGGGA84150.068.2081451
GCCGGGT1750.067.996631
GCGGGGT2600.067.304351
CTGGAAG15800.067.117583
CCGGGGA324750.066.935871
Produced by FastQC (version 0.11.5)