FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_3BRS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_3BRS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916342
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA489252.5530411586240866No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA467102.437456362173349No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166820.8705126746687177No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161360.84202089188673No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148500.7749138723672497No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA145650.7600417879480802No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128020.6680435955586216No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95960.5007456915310524No Hit
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78860.41151318501603573No Hit
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC60450.31544473794343597Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT48190.25146868356483343Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC46100.24056248832410917Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA43310.2260035004190275Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43250.22569040390493972No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC37520.19578968680955697No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC33930.17705607871663825No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT30970.16160998402164123Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC27740.1447549550132492Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25890.13510114582887606No Hit
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23750.12393403682641199No Hit
GGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA23230.12122053370431793Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC22900.11949850287683514Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG22560.1177242892970044Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG21110.11015779020654977Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG20960.10937504892133032Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG20900.10906195240724256Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC19990.10431332194357791Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA19650.10253910836374718Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGTG207.931047E-569.9980740
TCGATTA150.002225605369.996259
ATAAGGT150.002225605369.996256
AAATTCG150.002225605369.996255
TTACCAC150.002225605369.9962569
AATTCGA150.002225605369.996256
GGCAATG252.8426239E-669.996253
TTTCTAT150.002225605369.9962511
TGCCGCT150.002225605369.9962562
CTGGGAG252.8426239E-669.996253
CCGCTAT150.002225605369.996251
GGCGATA207.932072E-569.996251
TTATATG150.002225605369.9962513
TCGTGAG207.932072E-569.9962510
TCTATCC207.932072E-569.9962513
GTGAGTG207.932072E-569.9962512
CGGGCCT150.002225605369.9962570
CGGGTAC207.932072E-569.996252
TCACGTT150.002225605369.9962570
TATCCTT150.002225605369.9962515
Produced by FastQC (version 0.11.5)