FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_3BFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_3BFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14907951
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1575101.0565502931958926No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1442690.9677319170152894Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260240.8453475598356877No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1026960.6888673030921553Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC876620.5880217878365712Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA781900.5244852226841905No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG753900.5057032988638076Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT734140.492448626910566Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT609300.408708077991402Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA585990.3930721264109333No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA535900.35947260626225563No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA532820.3574065946420135No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470560.3156436454614051No Hit
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC462040.30992857435606Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA406710.27281415132099646Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA352580.23650466787823488Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG348110.2335062678969095Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA316480.2122894018098128Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA314970.21127651948949924No Hit
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC311590.2090092729711816Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT287210.19265558358757684Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA271670.18223161586726438No Hit
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA259020.17374621099841286Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC257290.17258575641951063Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG252300.169238549281521Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG230710.15475634445001865Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC227800.152804365938686Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA226780.1521201672852292Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT216450.1451909789614951Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA214760.1440573557023363No Hit
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT207740.1393484590873689Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC207030.13887220316192345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC191250.12828724752315057Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC185340.12432292003106261Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA184050.12345760996933784Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA175560.11776266235380033Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA168660.11313425969806314Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC166300.1115512118332023Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT160390.10758688434111434Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA159780.10717770671502744Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA155260.10414576758402277Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGCC150.002225656869.99818443
ATGGCGT207.933005E-569.9977269
TGTACAT150.00222571669.9977266
GCCTTTT252.8434406E-669.996792
GGCCTAT252.8434406E-669.996791
CTATGCG207.9335325E-569.9967870
CGTGCCT150.002225834469.996782
GCTGGAT150.002225834469.996782
CTCTTAG150.002225834469.996788
TGTGCCG150.002225834469.9967870
GGCCCTG150.002225834469.996781
CGCCTTG353.7198333E-969.996781
TTGAGGG150.002225834469.996788
GGGACTT150.002225834469.996782
GCCTCAT207.9335325E-569.996782
TGCCTGA150.002225834469.996784
GGTGCCT150.002225834469.996782
CGGTCAT150.002225834469.996782
GGTTCAT150.002225834469.996783
GGCCTGT150.002225834469.996781
Produced by FastQC (version 0.11.5)