FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_3ARS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_3ARS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502964
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC425361.6994251615284919Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC240830.962179240292709Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA225030.8990540814809961No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181500.7251402736915114No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146330.5846268663872114No Hit
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA144690.5780746347130842Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT109080.43580331159377445Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG105020.41958254293709374Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA99750.3985275057891364Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC96540.3857027108659973Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT95730.382466547661093Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA90890.363129473695986Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89010.3556183788500354No Hit
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC82600.3300087416359165Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA76550.30583739917953273Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG75630.30216175702087605Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT69840.27902918300063445Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67850.27107860920093135No Hit
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC67210.26852164074273543Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67080.26800225652466436No Hit
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC64810.25893300902450056Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC63960.25553703529095906Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC61730.24662759831943248Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT59420.2373985402906314Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56880.2272505717221662No Hit
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA50390.20132131345077275Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA49330.1970863344418857Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA46630.18629912375887148Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45550.18198423948566578No Hit
CGGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG41430.16552375503602929Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA40600.1622076865668064Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC38350.1532183443309612Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC35540.14199165469419456Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT34430.13755691252451094Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC33260.13288245456187145Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA33060.1320834019186852Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31450.125651028141036No Hit
CGGGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT30190.1206169964889627Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC29490.11782031223781086Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG28960.11570282273336732Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT28170.11254656479278169Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC26970.10775224893366424Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC26450.10567471206138002Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA26170.10455603836091928Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC26160.10451608572875999Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25700.10267826464943164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGTT401.3460522E-1069.995751
GACGTTG252.8429986E-669.995755
CGCTCTA207.932826E-569.995751
GGGCGGT252.8429986E-669.995752
GTGTCCG207.932826E-569.995753
GGACGCT207.932826E-569.995752
GGCATGG207.932826E-569.995751
CGCGTTG207.932826E-569.995752
GGGGCTT207.932826E-569.995753
GGGTGTC150.002225747869.995741
GCAAGTA301.0252188E-769.995746
AGTTTAG301.0252188E-769.9957412
GGTAAGT150.002225747869.995742
GGATGCT150.002225747869.995742
GGTCCAC150.002225747869.995742
GCTGCGT150.002225747869.995742
ATTCGTC150.002225747869.995747
TTCGTCT150.002225747869.995748
TAGCCTA150.002225747869.995748
GGCCAAT150.002225747869.995741
Produced by FastQC (version 0.11.5)