FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_3AFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_3AFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18056121
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2704491.4978244773614444No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2145161.1880514092700198No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1606850.8899198227570584Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1376090.7621182866463955No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1009840.5592784851187029Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA984370.5451724653373778No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA974640.5397837110196593No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC969860.5371364093096186Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA898570.4976539534709587No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG779390.43164863593902586Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT737920.40868135520358995Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA727680.40301014819295905No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT598190.33129485563372113Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA553930.30678239251941214No Hit
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450260.24936695982487045No Hit
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA437840.24248840600924199Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC433500.24008478897543942Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA364900.20209213263468936Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG352590.19527449998812035Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA346450.19187399109698036Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA336280.18624155210302368No Hit
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC308700.1709669535333752Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT282830.1566394022281973Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA270050.1495614700411013Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG249170.1379975244959867Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC242970.13456378587626877Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC234710.12998915990870907Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT232920.12899780633946792Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG224570.12437333577904135Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC222550.12325460158358487Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT216940.12014762196154977Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA212180.1175113968277018Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA209140.11582775724642076Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204400.11320260868876543No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC200280.11092083399308189No Hit
CGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC193230.10701634088517682Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC189660.10503917203479086No Hit
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA187160.10365460001071104Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTG150.002224603470.0066120
TTTGTGC150.00222553269.9992417
GTACCCT150.002225825369.996913
ATAGGTT207.933504E-569.996919
GGGCTCT353.7198333E-969.996911
GTGGCTA150.002225825369.996913
GTAGCTT207.933504E-569.996913
GTGCTCG600.069.996913
CTTGCTC252.8434333E-669.996913
GTGCGCT150.002225825369.996913
GCTGAGT150.002225898769.996332
GGTGCTT207.933831E-569.996332
CCGGGAA717900.067.890861
GCCGAGA22950.067.556931
GCCGGGA84500.067.511811
CCGGGGA498450.067.167261
GCCGGGT2200.066.815231
GCCGTGA6150.066.582421
GCCGAGT8100.065.676121
CGCTTGT800.065.621562
Produced by FastQC (version 0.11.5)