FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_2BRS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_2BRS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2532829
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740252.922621306057377No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA705762.7864494602675505No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA256031.010845974994759No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255871.0102142702882824No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216520.8548543940392345No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212130.8375219961552872No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198230.782642649780147No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA149200.5890646387892747No Hit
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121840.48104313398180454No Hit
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC101920.40239589802548853Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT79000.3119041988227393Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA75970.29994129094384187Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC75550.29828306608934124Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65500.2586041142137902No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT50500.19938179798162448Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC48490.19144600760651428Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43580.17206056942651873No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC42940.169533750600613No Hit
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40940.16163744176965758No Hit
GGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA40820.16116366323980022Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC39230.15488609771919068No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG39050.1541754299244047Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG37500.14805579058041424Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG36750.14509467476880594Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC35520.14023844483776834Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG35290.1393303693222085Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC34790.13735629211446965Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA32430.12803864769394224Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA28780.11362788407744857Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27800.10975869275028041No Hit
CGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA27540.10873217260225622Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26910.10624483532050526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACG455.456968E-1269.996671
CCGATGG150.002225634969.9966661
CGACGAT150.002225634969.9966663
CAGTTAA150.002225634969.9966665
GCTATGT500.069.99666670
GCAGTTA150.002225634969.9966664
GCCGGGT150.002225634969.9966661
GATTTAG150.002225634969.9966666
CGCGATA301.02512786E-769.9966661
CTGACTG150.002225634969.9966668
GCCGAGT750.069.9966661
CGGATGT150.002225634969.9966662
GGCGGTG150.002225634969.9966661
CGCAGAG600.069.9966661
GGCGCGA150.002225634969.9966661
GACGATA301.02512786E-769.9966664
GGCGAGT301.02512786E-769.9966661
GACCTTG150.002225634969.99666666
GCCGTTG150.002225634969.9966661
CGGAGAT150.002225634969.9966662
Produced by FastQC (version 0.11.5)