FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_2BFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_2BFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5695320
Sequences flagged as poor quality0
Sequence length76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1487402.6116179600092706Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC813661.428646678325362Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG456160.8009383142650458Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA388960.682946700097624No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG343770.6036008512252165Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA316220.5552278010717572Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA308210.5411636220616225No Hit
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT242950.42657831342224845Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC236870.4159028816642436Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT229000.4020845185169578Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT228690.40154021196350687Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC212180.3725514984232668Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT204290.3586980187241455Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA201800.3543260080206204No Hit
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA163470.28702513642780386Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT152670.2680621984366111Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC151980.2668506773982849Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG151050.26521775773793216Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140850.2473083163018057No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135550.23800243006538704No Hit
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA135520.23794975523763368Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC131750.231330285216634Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130460.22906526762324153No Hit
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA124790.21910972517786534Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA124350.2183371610374834Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC123450.216756916204884Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA122940.21586144413307767Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC122470.21503620516494246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT121900.21403538343762948Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT119850.21043593687448642Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114480.20100714270664335No Hit
CGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC106170.18641621541897557Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC100100.17575834193688855Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG96580.1695778288138331Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA93940.16494244397154154Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT89280.15676028739386022Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT83280.1462253218431976Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG81690.14343355597227198Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79150.1389737538891581No Hit
CGGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA71960.1263493535042807Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC71690.12587528005450088Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC69570.12215292555993343Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC68260.11985279141470541Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64380.11304018035861023No Hit
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA63510.11151261035376415Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC60470.10617489447476174Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC60080.10549012171396867Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC57700.10131125204553915Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGTG150.00222518870.0013438
TCTACTG150.00222565369.9976613
CTCTACT150.00222565369.9976612
TGCGGGA252.8431514E-669.997051
GCCGATA650.069.997051
GCCGAGT1650.069.997051
GCCAGTG252.8431514E-669.997051
TTAGAGG150.002225730369.997047
TATTGGA150.002225730369.997041
ATTTTTG150.002225730369.997041
GGGGTGT150.002225730369.997041
GAGGTAC150.002225730369.997044
CGCAGTG207.932966E-569.997041
TTTTGGA150.002225730369.997041
GAATTAG207.932966E-569.997044
TATACTA207.932966E-569.9970469
ACCTTGC207.932966E-569.9970469
ATTTCTA150.002225730369.9970410
CATGGCG150.002225807969.9964370
TGCCATA252.8432987E-669.9964370
Produced by FastQC (version 0.11.5)