FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_2ARS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_2ARS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2875257
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA581592.0227409236809093No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA465591.619298727035531No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA328901.1438977454884902No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205430.7144752625591382No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA186540.648776787605421No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177040.6157362628801529No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC165840.5767832232040475Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146300.5088240807691278No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124630.4334569048958058No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC100080.34807323310577104Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG95440.33193554523995594Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85970.29899935901382035No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG79000.27475804771538687Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68510.2382743525187488No Hit
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT65190.22672755861476035Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA48340.1681241016020481Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC45570.15849018018215416Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44760.15567304070557866No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT42960.14941273075763314Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA40900.14224815381720662Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG40650.14137866632443638Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38330.13330982239152883No Hit
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA35230.1225281774811782Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34580.12026750999997565No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC34290.11925890450836221No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC30960.10767733110466299No Hit
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA30880.10739909510697652Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC30720.1068426231116036Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT30630.10652960761420632Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGT150.00222485970.0030638
GCCGGTG150.00222547369.998191
CGCAGTG252.8425166E-669.998191
GCCGATT207.9316545E-569.9981841
GAGTGCC150.002225626569.9969750
GCGGTTC150.002225626569.996974
TGAAGGA150.002225626569.996974
GCAACAT207.932338E-569.996973
CGTGAAG207.932338E-569.996972
GCTATCT150.002225626569.9969770
GCAGTGA252.8428094E-669.996972
TGAGTAG150.002225626569.996978
TTTCCCA150.002225626569.9969766
TTTTTAG207.932338E-569.996972
TTCCAGC150.002225626569.9969750
GGCGGTT150.002225626569.996973
GTCTTCT150.002225626569.9969770
TTTTGAG401.3460522E-1069.996972
CCGGGAA120050.068.044891
GCCGAGT1750.067.998241
Produced by FastQC (version 0.11.5)