FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_2AFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_2AFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15752812
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC8468955.3761512547727985Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC4589252.913289386047393Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1726051.0957091343437604Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC1201290.7625876573655548Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1109190.7041219053461693Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT1027490.652258149211709Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT1026710.6517629995203396Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA920910.5846003875371584Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG914740.5806836265169673Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC904420.5741324152157723Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA887260.5632391220056457Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC749710.47592137835454396Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT681460.43259578035972246Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC673940.42782202948908427Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA671690.42639371307167256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT656390.416681161433273Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA652460.4141863687575272No Hit
CGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC611420.38813387730393784Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC589460.3741935090699997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA537390.3411390931346099No Hit
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA455620.28923090048938566Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC450250.2858219853064964Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC435820.2766617160161627Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA426560.2707834004493928Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370830.23540558980834658No Hit
GCCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT370680.2353103687138525Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT364220.23120951357763933Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC337270.21410145693353033Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC326210.20708048823283107Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA318070.20191315683828387Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG288930.18341487221456082Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC285670.1813454004275554Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT275690.17501002360721374Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA267220.1696332058047795Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC266030.16887778512179286Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT255300.16206630282898063Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT255090.16193299329668887Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC254850.1617806395454983Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA252460.16026345010655876Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAAAAAAAAAAAAAAAGAACGGAAGAGCACACGTCTGAACTCC252080.16002222333384034Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC247590.1571719385719832Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG241230.1531345641654328Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG237940.1510460481595286Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC236260.1499795719011945Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA233010.1479164481871554No Hit
CCCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT232410.14753556380917895Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC225390.14307921658685446Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC222420.141193838915871Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT222300.14111766204027573Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA221640.14069868922450163No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA218970.1390037537425064No Hit
GGCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT207360.13163364102866207Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC204700.12994505361963313Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT198020.12570454087816194Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA195960.12439683784710945Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC187790.11921046223366343Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186400.11832808009135132No Hit
GGGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG181220.11503977829482126Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT178900.11356702536664565Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT177190.11248150488941276Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAGAAAAAAAAAAAAAAAGGTCGGAAGAGCACACGTCTGAACTCC175370.11132615560955085Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA173630.11022159091341914No Hit
CCGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC170720.10837430168023335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC167130.1060953434853409Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC164960.1047178116516594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC158710.10075026604773803Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACACC158610.10068678531807526Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG150.002225780969.997229
GAGTGTG150.00222583769.996784
TGGAGCG150.00222583769.996784
GCATGTG150.00222589369.996342
GTGCTCG3100.068.867583
GCCGGGA180300.068.1913151
CTTGCTC5400.067.404093
CGCGGGA180250.066.695751
CCGGGGA710350.066.552641
GCCGAGA14300.066.32541
TTGCTCG14550.065.9076164
GTTGCTC9100.065.765993
CCGGGAA254300.065.152121
GCGTGGG9200.065.051151
CTGCTCG2750.064.905893
GCCGGAT1350.064.811621
GCCAGGG15650.064.629411
GGCTGGG6750.064.293131
GCCGAGG13400.064.2505651
GGCGGGA155350.064.003931
Produced by FastQC (version 0.11.5)