FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_1BRS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_1BRS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372031
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA441333.2166182834061328No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA419673.0587501302813127No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA152511.1115638057740678No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148121.079567444175824No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA138111.0066099089597829No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135180.9852547063440986No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117320.8550827204341592No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85860.6257876097551731No Hit
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70280.5122333241741622No Hit
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC46830.3413188185981221Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA45780.33366593028874714Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38120.27783628795559284No Hit
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT37100.2704020535979143Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC34550.25181646770371807Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC33700.24562127240565262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28760.20961625502630774No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC24840.1810454720046413No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT24050.1752875846099687Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22020.16049200054517718No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC21800.15888853823273671No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC21670.15794103777538554Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG21050.15342218944032607Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG19060.13891814397779642Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG18950.1381164128215762Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG17780.12958890870541556Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA17390.126746407333362Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA16840.12273775155226085Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16610.12106140458925492No Hit
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA15890.11581370974854067Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15680.11428313208666568No Hit
CGCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC15350.11187793861800498Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14340.10451658891089195No Hit
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA13920.10145543358714199Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTTTA150.002225143669.9988316
CTGGGAG207.931248E-569.9962843
GGCAAGG207.931248E-569.9962841
CGGGTTT207.931248E-569.99628470
GCCAGGG207.931248E-569.9962841
GCCGAGG353.7161954E-969.9962841
TAGGCCG207.931248E-569.99628450
CAATGAT150.002225465169.996287
GCCTTCC150.002225465169.9962870
AGTAGTT150.002225465169.996286
TGATCGA150.002225465169.9962810
GCGGTGA150.002225465169.996281
GCGCGTT150.002225465169.9962870
GGCGAGG150.002225465169.996281
ACCAAGT150.002225465169.9962848
TTCCCGC252.8421837E-669.9962869
GCAGTGA150.002225465169.996282
GCCGGTG252.8421837E-669.996281
GCTGTCC150.002225465169.996282
CCATCCT150.002225465169.9962870
Produced by FastQC (version 0.11.5)