FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_1BFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_1BFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5007245
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780261.5582620782486176No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA648341.2948038292514146No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA497920.9943991156813776No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC371580.7420847192418186Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320970.6410111748077036No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA269140.5375011608179747No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248400.49608117837253823No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA231330.46199057565587465No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC222290.4439367356700141Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150440.30044465569389955No Hit
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135530.2706678023543885No Hit
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC117830.23531902273605546Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113790.22725071371582578No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG111270.22221800610914785Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT89120.1779821039314034Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG84380.1685158205759854Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA83150.16605937995844022Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT79160.15809092624786686Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA75370.15052189377591868Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC72670.14512970705447806Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA72160.1441111828959837Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64410.12863360989925599No Hit
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA60990.12180350671876451Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC59930.11968657415405079No Hit
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT59210.11824865769499994Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC57950.11573230389166098Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56800.1134356317695659No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC56760.11335574752184086No Hit
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC56420.11267673141617796Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC54400.10864257690606312Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT54390.10862260584413184Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT52590.10502781469650477Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC51250.1023516923977157Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCA252.840925E-670.00606520
CTCGATC252.841094E-670.0053619
TCGTGAC150.002225018370.0025641
ACTCGAT252.8419363E-670.0018718
TAGCATT150.002225723469.9969711
TTAGGTT207.933301E-569.996287
TGATAGG150.002225811669.996285
GTTCTAG150.002225811669.996287
CGACGAT207.933301E-569.996283
CTATCGT150.002225811669.996286
GACTTGG252.843286E-669.9962810
TCAATCG150.002225811669.996288
GCTTCCT150.002225811669.996283
GCAATGT150.002225811669.996283
CGATATC150.002225811669.996283
GTACGTT150.002225811669.996283
GGCCTAT252.8434551E-669.995581
GCTGGAG207.933694E-569.9955752
GGTTTGC150.002225899669.99557570
GCGTTTC150.002225899669.99557570
Produced by FastQC (version 0.11.5)