FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_1ARS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_1ARS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758100
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC297651.6930208748080315Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264361.5036687332916217No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216151.229452249587623No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC177301.0084750583015756Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA149510.8504066890393038No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG114910.6536033217678175Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100440.5712985609464762No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93700.5329617200386781No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87390.49707070132529435No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG83140.47289687731073315Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73580.4185199931744497No Hit
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA61580.35026449007451227Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC57700.3281952107388658Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52550.2989022239918093No Hit
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT51790.29457937546214663Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT49360.2807576360844093Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44800.2548205449064331No Hit
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC44240.2516352880951027Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT42710.24293271144986064Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT42570.242136397247028Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA40120.2282008986974575Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA36090.20527842557306183Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA35980.20465275012797904Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34950.19879415277856777No Hit
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT34630.19697400602923612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA33860.1925942779136568Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA32420.1844036175416643Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC32170.18298162789374894Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG30950.17604231841192194Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA30020.1707525169216768Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT29840.16972868437517774Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC28550.16239121779193447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC26900.15300608611569308Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT26780.1523235310846937Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC25740.14640805414936578Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC24300.1382173937773733Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC23630.1344064615209601Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23190.13190375974062907No Hit
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA22140.12593140321938456Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT21240.12081224048688927Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC21020.11956088959672374Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC20050.11404356976281213Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG19650.11176838632614755Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC19060.10841249075706728Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG18230.10369148512598828Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCT252.840161E-670.0060621
TGAATTG207.928617E-570.00207517
TTAGACA207.931968E-569.9961111
CGCTGGA207.931968E-569.996111
TCCGGGA207.931968E-569.996111
GCCGGAT353.7161954E-969.996111
GGCTCGC207.931968E-569.9961170
GGGATGG207.931968E-569.996112
CGGCTTC207.931968E-569.9961170
CGAGAGT353.7161954E-969.996113
GCCGAGT650.069.996111
CGCTGGG1550.069.99611
TTTTGAG252.8425602E-669.99612
ACTGATT150.002225592569.99616
AGTAGTT150.002225592569.99616
GGCGTGG150.002225592569.99611
GAAGTAG150.002225592569.99614
AGGGTCA150.002225592569.996151
GGCGAGT550.069.99611
CTGACTA150.002225592569.99613
Produced by FastQC (version 0.11.5)