FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_1AFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_1AFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7774859
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1189791.5303042794731068No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA969781.247328086592953No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA621590.7994871675486335No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC517630.6657741317238036Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA419670.5397782776510802No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA418240.5379390160001616No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA414390.5329871577092267No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG368190.47356485821800753Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC318970.41025824391156157Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA318900.4101682101244537No Hit
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT303590.3904765346869956Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG279290.3592219485909648Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252390.32462325040235457No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT221000.2842495278692514Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193110.2483774946915436No Hit
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA176480.2269880392686221Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC170880.2197853362999895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA152360.19596496862515447No Hit
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA145930.18769472218081382Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG140870.18118656556987078Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC130570.16793873689542152Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA107930.1388192377508068Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA103510.13313424719342176Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG100290.12899269298645802Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC99220.12761646224066572Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA96590.12423376423932575No Hit
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT95290.12256170819303604Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG92320.11874170322574337Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC90970.11700533733151947Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC84410.10856788528254982No Hit
CGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC82790.1064842462094811Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC79780.10261279336384106No Hit
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA78450.10090215140879083Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTT150.002225659369.9978569
GATTTGG150.002225716169.997415
TTCTATC150.002225772769.9969512
TTTCTAT150.002225772769.9969511
TACGTTC150.002225772769.996958
GCTGGTT150.002225829569.99652
CCGATCG252.8433788E-669.99651
GCGCGTT207.933451E-569.99651
GCGCGGT150.002225829569.99651
CTGAGTG150.002225829569.99653
CGTGACT207.933451E-569.99652
GGCCGGT150.002225829569.99651
GGCCGAT252.8433788E-669.99651
TGAGTGA252.8433788E-669.99654
CGCTGAG252.8433788E-669.99651
GGGCGTT150.002225829569.99651
CTGTTTC150.002225829569.99656
CGTACTT150.002225829569.99652
CCCTTGA207.933451E-569.99652
GTATCAT207.933451E-569.99653
Produced by FastQC (version 0.11.5)