FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_0BRS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_0BRS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721613
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA271001.574105214121873No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA228881.3294509277055877No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC221641.2873973419113356Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA157090.9124582586214206No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC128960.7490649756943053Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA106750.6200580502122138No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94310.5478002315270621No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88780.5156791915488557No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79290.4605564665229643No Hit
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT57750.3354412402787386Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG56960.33085252028185197Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54440.31621508434241613No Hit
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT47140.27381298816865346Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA45500.26428703779536983Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45100.2619636352653006No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG44800.26022108336774874Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC43230.25110172843722717Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT40300.23408280490447042Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC36250.2105583542875199Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35240.20469176289909524No Hit
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA34490.2003353831552155Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC33680.19563049303182536Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA33450.1942945365770356Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC32430.1883698601253592Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT30610.1777983786135444Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA28870.16769157760774342Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC26160.1519505254665247Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25790.14980137812621072No Hit
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA25550.14840733660816918Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA24840.1442832971172964Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT23290.1352801123132783Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA22220.12906501054534325Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA21500.1248828859912187Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC20800.12081693156359762Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19940.1158216161239489No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT19500.11326587334087278Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19470.1130916181511176No Hit
GCCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT19240.11175566169632781Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC18110.10519204954888237Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18060.1049016242326237No Hit
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG17510.10170694575377859Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCT150.00222512269.999773
AGGCAGA150.00222512269.9997739
GTGGAAC150.00222512269.999773
CGCTGGG353.7161954E-969.9977341
TTTTGAG207.931006E-569.9977342
TTGTAAT150.002225378369.99773411
TAAAATG150.002225378369.99773464
GGCGACG301.0248186E-769.9977341
GGCTGGA150.002225378369.9977341
TCTATAT150.002225378369.99773411
GCCAGGG600.069.9977341
TCCGGGA150.002225378369.9977341
GCCGGTG150.002225378369.9977341
CGGCGAT252.8421418E-669.9977342
GACGTTG207.931006E-569.9977345
GAGTATA150.002225378369.9977345
GCCGGGT150.002225378369.9977341
GATTTGA150.002225378369.9977345
AGCACCT150.002225378369.99773412
CGCGGCG207.931006E-569.9977341
Produced by FastQC (version 0.11.5)