FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_0BFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_0BFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10214267
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC3365593.2949892537565346Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1919551.8792831634418798Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA722350.7071970998995816Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT680630.6663522698202426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG613010.6001507499265488Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT595010.5825283400169586Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC487370.4771463287576093Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT472210.46230434352264344Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC468380.4585546863029917Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG436700.4275392448621129Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA417530.40877137830839944No Hit
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA394550.3862734349904893Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT392870.38462867673226087Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA384070.37601327633201675Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC348810.3414929333646751Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC343860.3366467706395378Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA339900.332769840459428No Hit
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC292460.2863250001199303Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA275970.2701809145972002No Hit
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA272140.26643125737754847Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC269510.2638564274852028Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA230650.22581160253594312Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT229130.2243234879213555Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA223440.21875284834437947Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA222580.21791088875981018Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA216930.21237941009374436Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC213330.20885492811182632Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC187400.18346886761428893Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC186260.1823527816533482Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA176010.17231779823260934No Hit
CGGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC168310.16477932288239577Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT167280.1637709294264581Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT166130.16264505323778986Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT161120.15774014914628726Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA157660.15435273035255492No Hit
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT155040.1517876906879368Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC153350.15013314220198082Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC152600.14939887512241457Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG148890.14576670063549346Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC147250.14416110328817527Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT147150.14406320101089976Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC145970.14290795413904883Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA138740.13582961949203012Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135450.13260863456966615No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA133630.130826813123252No Hit
GGCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT133310.13051352583597042Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC130660.12791911548816962Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG130030.127302331141334Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC126300.12365057619895779Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT116600.11415405530323418Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC113470.111089714024511Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT113310.1109330703808702Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG112850.1104827199054029Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC110350.10803516297351538Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT106730.10449110053614225Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC104700.10250368430744956Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA102340.10019319056374776Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGAAT150.0022255469.99895542
TAATGTG800.069.99689512
GGGCTGG207.933925E-569.995871
GGATGTT207.933925E-569.995873
GGCTTGG207.933925E-569.995875
TGTTAGG401.364242E-1069.995875
GCGTGGC252.8435952E-669.995871
ATTTGTG252.8435952E-669.995871
GTGCGAT252.8435952E-669.995874
GCGCATT150.002225929269.9958651
GTGGCCT150.002225929269.9958653
CTGCGAT150.002225929269.9958653
CTGGGAT150.002225929269.9958653
CGTGGCT150.002225972269.995532
GCCTTTT150.002225972269.995532
TTTGTGA252.8436789E-669.995532
TTTGGAT150.002225972269.995532
GCAGAGT252.8436789E-669.995532
CGTTTTC252.8436789E-669.9955370
TTTTACG150.002225972269.995532
Produced by FastQC (version 0.11.5)