FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_0ARS.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_0ARS.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112647
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC303081.4345983971766225Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252651.1958931141832971No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206020.9751747452366628No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC189750.8981623527262246Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147490.6981289349332851No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108140.5118697065813645No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103470.4897647358976677No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85850.40636225550222066No Hit
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT84290.39897815394621067Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83580.39561744105853935No Hit
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG80080.3790505465418501Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC78340.37081443326783886Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG62740.2969734177077382Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT62520.29593207005240346Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA60410.2859445993580565Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT57720.2732117575723725Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48170.2280078025339775No Hit
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC46400.21962968730696608Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC46310.21920368144796554Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA45400.2148962888736263Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44880.21243492168828965No Hit
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA44690.21153557598595504Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA42380.20060142560494018Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC40690.19260198225259592Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT40330.1908979588165936Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39640.18763191389758913No Hit
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA36700.17371572250357017Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC36500.1727690428169022Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA35630.16865098617989657Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA31910.15104274400787257Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC31570.14943338854053706Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC30490.14432131823253008Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29790.14100793932919223No Hit
CGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC29680.14048726550152485Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA29430.1393039158931899Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT29230.13835723620652196Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT28300.13395517566351595Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT27400.12969511707351017Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC26870.12718641590384006Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23540.11142419912081858No Hit
GGCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC23470.11109286123048479Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23090.10929416982581566No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGAC150.002225580669.996694
CTGTCTG150.002225580669.996697
CATTCTT150.002225580669.996694
CCTTTGA252.8426184E-669.996693
GCAGGTT150.002225580669.996697
GCATGGT150.002225580669.996693
ATTCTTT150.002225580669.996695
CCTCTTT150.002225580669.996693
CGGACGT252.8426184E-669.996693
TTGCAAG207.9320125E-569.996685
CCGTTTC207.9320125E-569.9966870
CGGGTAT150.002225789569.995031
CGTATGT150.002225789569.995032
CGGCATT150.002225789569.995031
GGACTCT150.002225789569.995032
GCAGTAG150.002225789569.995032
GCCGGGT301.0252006E-769.995031
AGGGTCC150.002225789569.9950351
CGATTGT150.002225789569.995032
GCGTTTA150.002225789569.995032
Produced by FastQC (version 0.11.5)