FastQCFastQC Report
Thu 6 Sep 2018
HH2YKBGX7_n01_0AFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_0AFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8492888
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1238811.4586439854146198Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1184131.3942607037794446No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA962701.1335366720955227No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC757910.8924055044644413Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG648900.764051050714433Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA607760.7156105202376388No Hit
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG489610.5764941207278372Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA428470.5045044748029175No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA417300.49135229382513934No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA405280.4771992754408159No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA337630.39754439243753126No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT335430.3949539897382374Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT319210.3758556571098077Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC301430.354920493476424Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA258170.3039837567621285Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA246920.2907373793225579No Hit
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA215260.2534591295681752Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA211100.24856091355496507Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT203460.23956515145378107Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA191560.2255534277621464No Hit
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA187600.22089070290341753Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC173700.20452406766697026Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT171650.20211028333353742Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG167190.19685883058860543Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC165800.1952221670649607Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA165510.19488070489096287Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA159790.188145657872799Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154380.18177562214408102No Hit
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA154010.1813399635082907Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT153060.18022138052450473Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA141990.16718694512396726Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC140790.16577399819707972Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT134860.15879168546671052Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT129530.15251584619978503Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG124980.14715842243533647Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA115770.13631405477147468No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG115150.13558403219258278Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC113260.13335864078273493Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC109930.12943771306062202Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC99590.11726282037394112Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA97730.11507275263726545Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC96310.11340076544044853Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT94240.11096343199156752Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC93380.10995082002729813Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA92730.10918547377523405Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC86560.10192057165948733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC86520.10187347342859109Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGT207.9331294E-569.9971569
GGTCGAT150.002225806269.996743
TAGGTCG150.002225806269.996749
GGCCGTT150.002225806269.996741
TCTGTCC150.002225806269.996748
GCCAGTG252.8433442E-669.996741
GCGTCCT150.002225806269.996741
GTGCTCG1050.069.9967353
CGGTCAG207.933361E-569.9967351
GGCGAGT1450.069.9967351
GGTACGT150.002225858269.996332
GCCGGGA59250.068.224671
CCGGGAA286400.066.9661561
CCGGGGA281950.066.781781
CGCGGGA52650.066.207751
GCCGGAG8700.065.973941
GTGGAAG26400.065.621943
CTGCTCG1550.065.480823
GCCGGGT750.065.330291
GCCGAGA9500.065.207491
Produced by FastQC (version 0.11.5)