FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n02_HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n02_HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24219733
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4021981.6606211141964282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACA8286100.043.8798261
TCACATC8271250.043.8080253
ATCACAT8306150.043.8002552
ATCGGTC8277700.043.7157867
CATCGGT8293250.043.7093356
ACATCGG8315250.043.694055
TCGGTCC8250350.043.6927078
CGGTCCC8240400.043.6618049
CATGTTA2337850.043.6576922
CACATCG8288600.043.650594
GGTCCCA9660200.043.58208510
CGTATTC2180000.043.5657927
CTAACGG2203200.043.5433276
GTATTCC2182400.043.5178838
AGCTTAC2481750.043.5131381
TATCCCC2445650.043.5096938
CCGTCCC2309550.043.5025189
ACTGTCT2215000.043.4760551
GTCCCAT12020150.043.4741911
TGTCTAA2214850.043.465473
Produced by FastQC (version 0.11.5)