FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n02_ADT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n02_ADT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40053088
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1946890.4860773781037807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACCA13797850.043.8237151
GCATTCT3955900.043.8180581
TAACCAG13823850.043.7892342
CCAGTTG13803150.043.724495
TTGTCCG8791250.043.7226075
GTACCCC13708900.043.71076611
TACCCCC13654550.043.70257612
TCCGATT8787500.043.7009248
TGTCCGA8798550.043.7003866
CTATTCA3535350.043.6933753
TTGTACC13737650.043.6837239
GTTGTCC8800050.043.6836664
GTTGTAC13801200.043.681318
ACCAGTT13833250.043.6712724
TGTACCC13735850.043.67118510
AGTTGTC8850000.043.6705363
CCGATTC8756750.043.6668479
TCTATTC3534150.043.6617662
TATTCAG3544100.043.6562654
GATTCCC8797150.043.6481611
Produced by FastQC (version 0.11.5)