FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32373093
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAAAAAAA423100.13069495707438275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGT372460.11505233682799479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAAA85850.019.10414118
GGGGGGT44600.018.41313721
CCGGTAG37350.018.1133231
CTTGAAA90600.017.91667417
CCGAGAA35150.017.2647045
GACTAAC30450.017.249861
ATGCCGT97300.017.146144
GGGAATG23850.017.1342091
CGTGTCC32450.016.98673610
GTACTCC31550.016.7816261
GACCTGG22350.016.592051
GCGCGAT19900.016.5759891
AGGACGA19150.016.4480518
TTAGGCA33800.016.4415151
GAAGGTT45600.016.4398829
AGACGTT29700.016.3767381
CTACCCA34600.016.3649831
CGTCTAC27600.016.0622441
GAGAAGG48200.016.0320387
TGCCAAA33050.015.98819351
Produced by FastQC (version 0.11.5)