FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_cDNA_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_cDNA_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78764942
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC222450.017.7495141
AGCTTGA241300.017.7341441
CTGATAG303400.017.663281
TGCCAAA304700.017.4431361
TATGCCC252600.017.4065381
CAATGGA260850.017.3925158
GAAATGA287750.017.3682351
GGGCATC256700.017.3576661
ACTGAAC190500.017.351951
TTGGAAC237300.017.2009181
CTACCCA326350.017.1775071
AGACGTT278650.017.158911
GGGAATG217300.017.048741
TTGAACG218550.017.0425451
TGATTTC266100.017.0129221
ACTGATG178450.017.0050331
AAATGCC287800.016.9783481
TGACGGC188400.016.9543841
GCGCGAT188200.016.871941
CTCGAAA164400.016.861031
Produced by FastQC (version 0.11.5)