FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_cDNA_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_cDNA_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75449369
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC207150.017.8939951
CTGATAG296600.017.7032341
AGCTTGA239500.017.639131
TGCCAAA299350.017.4879681
CAATGGA250000.017.4334558
GGGAATG203650.017.2731421
TATGCCC241250.017.2630081
GAAATGA272100.017.193421
CTACCCA323200.017.1756921
TTGGAAC222900.017.1479641
ACTGAAC175950.017.0613561
GGGCATC248100.017.0573311
TTGAACG207850.017.035281
ACTGATG174400.017.0323091
TGATTTC257300.017.0271131
TTTATGC254500.017.0204681
TGACGGC172950.017.0050561
ATTGGTG291550.016.9722981
AAATGCC275150.016.964661
AGACGTT259350.016.9451751
Produced by FastQC (version 0.11.5)