FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_cDNA_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_cDNA_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124340384
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC337050.017.934471
AGCTTGA392500.017.8815171
CTGATAG481700.017.8192421
CAATGGA409950.017.4799658
TGCCAAA486750.017.3840521
TATGCCC403350.017.3666551
ACTGAAC290550.017.3378731
CTACCCA531550.017.3357071
GAAATGA457850.017.2608871
GGGAATG334700.017.2130741
AAATGCC455500.017.1124271
TGATTTC421200.017.094511
TTGGAAC361050.017.082691
TTTATGC412100.017.0310481
TGCTACC355100.017.0258051
GGGCATC412050.016.9770361
AGACGTT432500.016.9368781
TGACGGC293900.016.9043751
ACTGATG290600.016.8758561
GACCTGG334450.016.8647791
Produced by FastQC (version 0.11.5)